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At metamorphosis (complete tail reabsorption—Niewkwoop–Faber Stage 66), the date was recorded for each animal.

Each animal was weighed to the nearest 0.002 g on a Mettler AT 261 Delta Range balance and its total length was measured to the nearest 0.5 mm.

Atrazine is the most commonly used herbicide in the U. We examined the effects of atrazine on sexual development in African clawed frogs ().

It can be present at several parts per million in agricultural runoff and can reach 40 parts per billion (ppb) in precipitation.

Food levels were adjusted as the animals grew to maximize growth. 1, we exposed larvae to atrazine at nominal concentrations of 0.01, 0.1, 1.0, 10.0, and 25 parts per billion (ppb), whereas the second experiment used 0.1, 0.4, 0.8, 1.0, 25, and 200 ppb atrazine.

We report results from two experiments that used frogs from two separate sources. 1 were from a long-term captive colony maintained at the University of California, Berkeley, whereas adults from Exp. In both experiments, three females and three males were injected with human choriogonadotropin (1,000 international units) 6 h before harvesting gametes.

Eggs were manually stripped from the female and fertilized in 0.3 × modified mammalian Ringer's solution by using the sperm obtained from the dissected testes of the three males. After 4 days, the larvae were all mixed and netted into tanks 5 at a time repeatedly, until all tanks contained 30 larvae.

Animals were anesthetized in 0.2% benzocaine (Sigma), assigned a unique identification number, fixed in Bouins' fixative, and preserved in 70% ethanol until further analysis.

Initially, the sex of all individuals was determined based on gross gonadal morphology (Fig. Sex identification was confirmed by histology for 10 animals per tank.

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